Coronary heart sections within the mid-ventricle ended up preset in paraformaldehyde and embedded in paraffin; morphology was determined by H&E staining. Cardiomyocyte cross-sectional location was resolute by staining with rhodamine-conjugated wheat germ agglutinin (Vector Laboratories RL-1022). Oil purple O staining was done to identified lipid accumulation. Take a look at https://cullenu099gsd2.wikikarts.com/user